ENHANCING SALT TOLERANCE OF WHEAT PLANT (TRITICUM AESTIVUM L.) BY APPLICATION OF PROLINE, ASCORBIC ACID, ARGININE, GLUTAMINE AND GLUTATHIONE

Salinity is becoming nowadays one of the major abiotic stress issues worldwide. Wheat is considered as moderately salt tolerant crop and is adversely affected in response to the salt stress in terms of growth and yield. Pot experiment was conducted to determine the effect of exogenous proline, ascorbic acid, arginine, glutamine and glutathione application on some growth and biochemical parameters of wheat grown under salt stress conditions. The experiment was conducted under field conditions of wheat (Triticum aestivum L.) cultivar sakha 93. Three replicates, five grains per replicate were sown directly in plastic pots. Wheat plants were treated with 100 mg/l proline, 88 mg/l ascorbic acid, 100 mg/l arginine, 10 mg/l glutamine and 50 mg/l glutathione. Salinity treatments were established by adding 6000, 8000 and 10000 mg/l of sea salt. The results obtained from this experiment revealed that growth parameters such as shoot length, root weight andgrain weight, in addition tobiochemical compounds such as chlorophyll, starch, fiber, ash and fat were affected by both salt and treatments. These parameters were decreased in response to salinity stress compared to untreated plants. The decrement in the growth parameters andbiochemical compounds were found to be increased with increasing salt concentrations, particularly at 6000 mg/l, 8000 mg/l and 10000 mg/l respectively. The selected compounds mitigate the negative effects of salt stress and improved growth parameters and biochemical compounds compared with control plants under different salinity level.
Key words: abiotic stress, salt stress, salinity tolerance and wheat plant.
Salinity is becoming nowadays one of the major abiotic stress issues worldwide. Wheat is considered as moderately salt tolerant crop and is adversely affected in response to the salt stress in terms of growth and yield. Pot experiment was conducted to determine the effect of exogenous proline, ascorbic acid, arginine, glutamine and glutathione application on some growth and biochemical parameters of wheat grown under salt stress conditions. The experiment was conducted under field conditions of wheat (Triticum aestivum L.) cultivar sakha 93. Three replicates, five grains per replicate were sown directly in plastic pots. Wheat plants were treated with 100 mg/l proline, 88 mg/l ascorbic acid, 100 mg/l arginine, 10 mg/l glutamine and 50 mg/l glutathione. Salinity treatments were established by adding 6000, 8000 and 10000 mg/l of sea salt. The results obtained from this experiment revealed that growth parameters such as shoot length, root weight andgrain weight, in addition tobiochemical compounds such as chlorophyll, starch, fiber, ash and fat were affected by both salt and treatments. These parameters were decreased in response to salinity stress compared to untreated plants. The decrement in the growth parameters andbiochemical compounds were found to be increased with increasing salt concentrations, particularly at 6000 mg/l, 8000 mg/l and 10000 mg/l respectively. The selected compounds mitigate the negative effects of salt stress and improved growth parameters and biochemical compounds compared with control plants under different salinity level.